6RSQ

Helical folded domain of mouse CAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	82.510, 91.410, 106.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.260 - 2.370
R-factor	0.188
Rwork	0.186
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s0p
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180409)
Data scaling software	XSCALE (VERSION Jan 26, 2018 BUILT=20180409)
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.260	2.455
High resolution limit [Å]	2.370	2.370
Rmerge	0.138	1.786
Rmeas	0.149	1.921
Rpim	0.056	0.704
Number of reflections	33463	3266
<I/σ(I)>	10.63	1.31
Completeness [%]	99.8	99.81
Redundancy	7	7.3
CC(1/2)	0.998	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.1	293	0.1M sodium cacodylate, 12% PEG4000 (w/v), pH 6.1