6RRX

GOLGI ALPHA-MANNOSIDASE II in complex with (2S,3R)-2-(Hydroxymethyl)-3-piperidinol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	89.005, 91.377, 132.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.340 - 1.840
R-factor	0.203
Rwork	0.200
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bub
RMSD bond length	0.008
RMSD bond angle	1.514
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.340	1.870
High resolution limit [Å]	1.840	1.840
Number of reflections	94351	4622
<I/σ(I)>	13.2	1.2
Completeness [%]	100.0	100
Redundancy	8.1	8
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M sodium succinate, pH 7.4 10 % PEG 3350 with microseeding