6RRV

Crystal structure of the Sir4 H-BRCT domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.999
Spacegroup name	C 1 2 1
Unit cell lengths	69.660, 38.130, 48.620
Unit cell angles	90.00, 118.09, 90.00

Refinement procedure

Resolution	42.892 - 1.100
R-factor	0.1184
Rwork	0.117
R-free	0.14170
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.249
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXCD
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.900	1.130
High resolution limit [Å]	1.100	1.100
Number of reflections	43703	2598
<I/σ(I)>	20.8	7
Completeness [%]	95.2
Redundancy	3.4
CC(1/2)	0.990	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	35% PEG 2000 MME 150 mM potassium bromide