6RRS
T=3 MS2 Virus-like particle
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | FEI FALCON I (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.9 |
| Software | RELION,EPU,CTFFIND,UCSF Chimera |
| Symmetry | I |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
