6RRR

Crystal structure of the tyrosinase PvdP from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	44.162, 112.616, 100.265
Unit cell angles	90.00, 92.71, 90.00

Refinement procedure

Resolution	49.130 - 2.110
R-factor	0.1717
Rwork	0.169
R-free	0.22000
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.544
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.110	2.110
Rmerge	0.080	0.657
Rmeas	0.091	0.742
Rpim	0.042	0.339
Number of reflections	55955	4391
<I/σ(I)>	12.2
Completeness [%]	99.4	95.7
Redundancy	4.5	4.5
CC(1/2)	0.998	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG 3350, 0.1M Bis-Tris Propane