6RRD
RNA Polymerase I Pre-initiation complex DNA opening intermediate 1
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.1 |
Software | SerialEM,Gctf |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |