6RR0
Crystal structure of the Sir4 H-BRCT domain in complex with Ubp10 pT123 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.040, 75.280, 95.910 |
Unit cell angles | 90.00, 97.24, 90.00 |
Refinement procedure
Resolution | 47.570 - 2.180 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.180 | 2.180 |
Number of reflections | 51736 | 3797 |
<I/σ(I)> | 9.51 | 1.02 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.8 | 6.8 |
CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 27.8 % PEG 4000 0.1 M sodium acetate pH 4.9 0.2 M ammonium acetate 3% w/v trimethylamine N-oxide dehydrate |