6RQP

Steady-state-SMX dark state structure of bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 63
Unit cell lengths	62.000, 62.000, 110.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.336 - 1.800
R-factor	0.1753
Rwork	0.174
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g7h
Data reduction software	CrystFEL (0.7)
Data scaling software	CrystFEL (0.7)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.800	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	22272
<I/σ(I)>	14.3	1.17
Completeness [%]	100.0	100
Redundancy	1587.9	198.2
CC(1/2)	0.990	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.6	294	100 mM Na/K Phosphate buffer pH 5.6 30 % PEG 2000