6RPY
Cytokine receptor-like factor 3 C-terminus residues 174-442: Hg-SAD derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.006 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.254, 40.944, 76.288 |
| Unit cell angles | 90.00, 98.73, 90.00 |
Refinement procedure
| Resolution | 27.310 - 1.970 |
| R-factor | 0.1887 |
| Rwork | 0.186 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.134 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.310 | 2.020 |
| High resolution limit [Å] | 1.967 | 1.967 |
| Rmerge | 0.067 | 0.714 |
| Rmeas | 0.079 | 0.845 |
| Number of reflections | 19156 | 1351 |
| <I/σ(I)> | 16.6 | 2.1 |
| Completeness [%] | 99.7 | 96 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.999 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 20 % PEG 3,350, 0.2 M sodium formate, soaked for 16 hours with 10 mM thimerosal, cryo-protected in 25 % ethylene glycol. |
