6RP7

The crystal structure of a complex between the LlFpg protein, a THF-DNA and an inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 41 21 2
Unit cell lengths	91.669, 91.669, 141.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.834 - 2.000
R-factor	0.1668
Rwork	0.166
R-free	0.18550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pm5
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.064
Number of reflections	41266	5925
<I/σ(I)>	13.5
Completeness [%]	99.8	99.9
Redundancy	5.8	5.8
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	HEPES, CITRATE