6RP7
The crystal structure of a complex between the LlFpg protein, a THF-DNA and an inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 91.669, 91.669, 141.043 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.834 - 2.000 |
R-factor | 0.1668 |
Rwork | 0.166 |
R-free | 0.18550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pm5 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.720 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.064 | |
Number of reflections | 41266 | 5925 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.8 | 5.8 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | HEPES, CITRATE |