6ROU
REP related 18-mer DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979491 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 38.563, 38.563, 89.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.442 - 2.902 |
| R-factor | 0.2721 |
| Rwork | 0.264 |
| R-free | 0.31150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Deposition ID D_1200013700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.015 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc3_3199: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.970 | 3.100 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.061 | 0.710 |
| Rmeas | 0.064 | 0.735 |
| Rpim | 0.019 | 0.188 |
| Number of reflections | 1758 | 299 |
| <I/σ(I)> | 20 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.1 | 14.7 |
| CC(1/2) | 1.000 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | Natrix crystallization screen (Hampton Research) precipitant 30-32% (+/-)-2-Methyl-2,4-pentanediol buffer 0.04M Sodium cacodylate trihydrate salt 0.04 M Lithium chloride 0.08 M Strontium chloride hexahydrate additive 0.012 M spermine tetrahydrochloride |
