6RNZ
Crystal structure of the N-terminal HTH DNA-binding domain of the essential repressor DdrO from radiation-resistant Deinococcus bacteria (Deinococcus deserti)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.257, 43.227, 45.870 |
| Unit cell angles | 90.00, 106.66, 90.00 |
Refinement procedure
| Resolution | 43.940 - 1.350 |
| R-factor | 0.13114 |
| Rwork | 0.129 |
| R-free | 0.17701 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rnx |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.375 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.048 | |
| Number of reflections | 31092 | 2968 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris propane pH 7, 2.5 M Ammonium Sulfate |
