6RNX

Crystal structure of the essential repressor DdrO from radiation-resistant Deinococcus bacteria (Deinococcus deserti)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.8726
Spacegroup name	C 2 2 21
Unit cell lengths	32.902, 130.188, 154.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.810 - 2.840
R-factor	0.2258
Rwork	0.222
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rmq
RMSD bond length	0.011
RMSD bond angle	1.194
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.940
High resolution limit [Å]	2.840	2.840
Rmerge	0.159
Number of reflections	8234	764
<I/σ(I)>	8.6
Completeness [%]	99.0
Redundancy	9.6
CC(1/2)		0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Bis-Tris pH 5.5, 0.2 M Na-thiocyanate, 2.3 M NaCl