6RNS

Crystal structure of the dimerization domain of Gemin5 at 2.7 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	2.0330
Spacegroup name	P 1 21 1
Unit cell lengths	67.851, 54.622, 103.032
Unit cell angles	90.00, 108.82, 90.00

Refinement procedure

Resolution	64.220 - 2.690
R-factor	0.235
Rwork	0.234
R-free	0.26450
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.433
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.530	2.787
High resolution limit [Å]	2.690	2.691
Rmerge	0.140
Rmeas	0.168
Rpim	0.091
Number of reflections	20115	1946
<I/σ(I)>	6.61
Completeness [%]	97.9	96.71
Redundancy	3.3	3.1
CC(1/2)	0.989	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	200 mM NaI, 25% PEG 3350 and 100 mM BisTris Methane pH 8.5