6RNS
Crystal structure of the dimerization domain of Gemin5 at 2.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 2.0330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.851, 54.622, 103.032 |
| Unit cell angles | 90.00, 108.82, 90.00 |
Refinement procedure
| Resolution | 64.220 - 2.690 |
| R-factor | 0.235 |
| Rwork | 0.234 |
| R-free | 0.26450 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.433 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.530 | 2.787 |
| High resolution limit [Å] | 2.690 | 2.691 |
| Rmerge | 0.140 | |
| Rmeas | 0.168 | |
| Rpim | 0.091 | |
| Number of reflections | 20115 | 1946 |
| <I/σ(I)> | 6.61 | |
| Completeness [%] | 97.9 | 96.71 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.989 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 200 mM NaI, 25% PEG 3350 and 100 mM BisTris Methane pH 8.5 |
