6RNQ
Crystal structure of the dimerization domain of Gemin5 at 1.95 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.219, 54.972, 103.640 |
Unit cell angles | 90.00, 104.30, 90.00 |
Refinement procedure
Resolution | 100.400 - 1.950 |
R-factor | 0.2118 |
Rwork | 0.211 |
R-free | 0.23540 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 0.874 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.400 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 51558 | 5087 |
<I/σ(I)> | 9.96 | |
Completeness [%] | 99.2 | |
Redundancy | 3.4 | |
CC(1/2) | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM NaK Tartrate, 25% PEG 3350 and 100 mM BisTris Methane pH 8.5 |