6RNH
Structure of C-terminal truncated Plasmodium falciparum IMP-nucleotidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 211.860, 211.860, 105.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 3.700 |
| R-factor | 0.2682 |
| Rwork | 0.267 |
| R-free | 0.29060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rmd |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.512 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 4.230 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 15185 | 4966 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 99.1 | |
| Redundancy | 45 | |
| CC(1/2) | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 2M ammonium sulfate, 0.1M Bis-tris pH 5.5 and 0.01M |
