6RNB
Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3 50ms diffusion time
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-04-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.700, 78.700, 37.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.649 - 1.699 |
R-factor | 0.1701 |
Rwork | 0.169 |
R-free | 0.18620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5njr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.157 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.649 | 1.830 |
High resolution limit [Å] | 1.699 | 1.699 |
Number of reflections | 13641 | 1334 |
<I/σ(I)> | 7.6 | |
Completeness [%] | 100.0 | |
Redundancy | 502.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | 20 mM NaAcetate pH 4.7, 50 mM NaCl |