6RMQ
Crystal structure of a selenomethionine-substituted A70M I84M mutant of the essential repressor DdrO from radiation resistant-Deinococcus bacteria (Deinococcus deserti)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 34.450, 76.980, 131.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.000 - 3.000 |
| R-factor | 0.20206 |
| Rwork | 0.197 |
| R-free | 0.28382 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.961 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CRANK |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 3.300 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.079 | |
| Number of reflections | 7531 | 1193 |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.5 M Ammonium Chloride 0.1 M Bis-Tris propane pH 7 |
