6RMN
DNA mismatch repair proteins MLH1 and MLH3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 163 |
Detector technology | PIXEL |
Collection date | 2015-06-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 92.260, 104.360, 135.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.140 - 2.200 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e4w |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | XDS |
Data scaling software | STARANISO (1.10.9) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.100 | |
Rmeas | 0.104 | |
Rpim | 0.029 | 0.420 |
Number of reflections | 33362 | 2168 |
<I/σ(I)> | 12.8 | |
Completeness [%] | 98.7 | 87.4 |
Redundancy | 13 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10% PEG4000, 200 mM imidazole-malate, pH6 and 10 mM MgCl2 |