6RMA

Crystal structure of the DEAH-box ATPase Prp2 in complex with Spp2 and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	66.670, 95.260, 124.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.549 - 2.100
R-factor	0.2252
Rwork	0.224
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.549	50.000	2.200
High resolution limit [Å]	2.100	17.000	2.100
Rmerge	0.053	0.027	1.144
Rmeas	0.060	0.031	1.291
Total number of observations	212720
Number of reflections	46827	98	5979
<I/σ(I)>	14.83	40.41	1.58
Completeness [%]	99.4	92.5	99.4
Redundancy	4.543	3.418	4.645
CC(1/2)	0.999	0.998	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	100 mM sodium cacodylate pH 6, 33 mM KCl, 12% (v/v) isopropyl alcohol, 25 mM MgCl2