6RLZ
Crystal Structure of 14-3-3zeta in complex with a macrocyclic 8-mer peptide derived from ExoS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97870 |
Spacegroup name | P 64 |
Unit cell lengths | 98.800, 98.800, 94.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.810 - 3.700 |
R-factor | 0.22336 |
Rwork | 0.216 |
R-free | 0.29220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.841 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 10.000 |
High resolution limit [Å] | 3.700 | 3.700 |
Number of reflections | 5633 | 186022 |
<I/σ(I)> | 16.02 | |
Completeness [%] | 99.1 | |
Redundancy | 33 | |
CC(1/2) | 0.992 | 0.921 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M Magnesium formate 20% PEG 3350 pH 7.5 |