6RLR
Crystal structure of CD9 large extracellular loop
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 39.986, 39.998, 63.643 |
Unit cell angles | 80.39, 76.29, 68.15 |
Refinement procedure
Resolution | 29.017 - 2.000 |
R-factor | 0.241 |
Rwork | 0.239 |
R-free | 0.27940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rlo |
RMSD bond length | 0.008 |
RMSD bond angle | 1.393 |
Data reduction software | EVAL15 |
Data scaling software | EVAL15 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.017 | 2.072 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.121 | 1.364 |
Rpim | 0.065 | 0.732 |
Number of reflections | 22863 | 2180 |
<I/σ(I)> | 4.82 | 0.82 |
Completeness [%] | 95.6 | 93.003 |
Redundancy | 3.407 | 3.355 |
CC(1/2) | 0.991 | 0.322 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | pentaerythritol propoxylate, potassium chloride, sodium citrate |