6RL2
The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.787, 120.031, 39.641 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.883 - 1.940 |
| R-factor | 0.1744 |
| Rwork | 0.172 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rkh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.085 | 0.645 |
| Number of reflections | 40538 | 5211 |
| <I/σ(I)> | 24.7 | 4.2 |
| Completeness [%] | 99.0 | 94 |
| Redundancy | 13.7 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4K, 0.1M Tris/HCl buffer, pH 9 |
