6RL1
The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.088, 120.230, 39.474 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.614 - 1.700 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.19960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rkh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.064 | 0.427 |
Number of reflections | 54441 | 7085 |
<I/σ(I)> | 29.5 | 5.5 |
Completeness [%] | 97.7 | 86 |
Redundancy | 13.7 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4K, 0.1M Tris/HCl buffer, pH 9 |