6RL0
Recombinant Pseudomonas stutzeri nitrous oxide reductase, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.36999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.916, 73.372, 136.342 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 48.910 - 1.780 |
| R-factor | 0.15876 |
| Rwork | 0.157 |
| R-free | 0.19225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sbq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.946 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 205189 | 205189 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M bis-tris propane buffer at pH 8.5, 0.1 M sodium formate, 0.1 M sodium chloride, and 25% (w/v) of a medium molecular weight (MMW) polyethylene glycol mixture (PEG 2K, 3350, 4K and 5K MME) |
