6RKU
E. coli DNA Gyrase - DNA binding and cleavage domain in State 1
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 0.01 |
Phase Plate | VOLTA PHASE PLATE |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4 |
Number of Particle | 1572962 |
Software | cryoSPARC,RELION,SerialEM,UCSF Chimera,PHENIX |
Symmetry | C2 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |