6RKS
E. coli DNA Gyrase - DNA binding and cleavage domain in State 1 without TOPRIM insertion
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4 |
| Number of Particle | 1572962 |
| Software | RELION,PHENIX,SerialEM,UCSF Chimera,cryoSPARC |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
