6RKJ
The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinooctaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.477, 95.608, 120.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.912 - 1.601 |
R-factor | 0.1603 |
Rwork | 0.159 |
R-free | 0.18770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rkh |
RMSD bond length | 0.006 |
RMSD bond angle | 1.144 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.053 | 0.332 |
Number of reflections | 60551 | 4532 |
<I/σ(I)> | 10.5 | 2.7 |
Completeness [%] | 99.6 | 95.1 |
Redundancy | 6.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG 4K, 0.1M Tris/HCl buffer, pH 9 |