6RK7
Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 113.999, 106.139, 234.377 |
| Unit cell angles | 90.00, 96.86, 90.00 |
Refinement procedure
| Resolution | 48.660 - 1.800 |
| R-factor | 0.17017 |
| Rwork | 0.169 |
| R-free | 0.19448 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rjs |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.154 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.033 | 0.566 |
| Number of reflections | 255563 | 12581 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.996 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Hepes pH 7.0 and 30% Jeffamine ED-2001 |
