6RK5

Inter-dimeric interface controls function and stability of S-methionine adenosyltransferase from U. urealiticum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	56.358, 80.450, 158.849
Unit cell angles	90.00, 94.46, 90.00

Refinement procedure

Resolution	47.600 - 2.600
Rwork	0.209
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rjs
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.099	0.394
Number of reflections	42328	13239
<I/σ(I)>	7.1
Completeness [%]	96.8
Redundancy	7.1
CC(1/2)	0.984	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M MgCl2, 0.1M Hepes pH 7.4 and 20% PEG 3350