6RK0
Structure of the Flavocytochrome Anf3 from Azotobacter vinelandii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.970, 89.870, 59.030 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 32.240 - 0.990 |
| R-factor | 0.1243 |
| Rwork | 0.124 |
| R-free | 0.13800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ybn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.332 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.240 | 1.025 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.035 | 0.728 |
| Number of reflections | 203126 | 5207 |
| <I/σ(I)> | 15.65 | |
| Completeness [%] | 76.0 | 19.54 |
| Redundancy | 3.2 | 2 |
| CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 25.5% PEG 4000, 170 mM ammonium sulfate, 15% (v/v) glycerol |
