6RJY
The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-15 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.406, 95.704, 119.935 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.438 - 1.621 |
R-factor | 0.1602 |
Rwork | 0.159 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rkh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.081 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.450 | 1.720 |
High resolution limit [Å] | 1.620 | 1.620 |
Rmerge | 0.085 | 0.773 |
Number of reflections | 58348 | 9134 |
<I/σ(I)> | 15 | |
Completeness [%] | 99.6 | 97.8 |
Redundancy | 6.8 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4K, 0.1M Tris/HCl buffer, pH 9 |