6RJY
The crystal structure of AbnE, an arabino-oligosaccharide binding protein, in complex with arabinobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.406, 95.704, 119.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.438 - 1.621 |
| R-factor | 0.1602 |
| Rwork | 0.159 |
| R-free | 0.18790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rkh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 1.720 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.085 | 0.773 |
| Number of reflections | 58348 | 9134 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 22% PEG 4K, 0.1M Tris/HCl buffer, pH 9 |
