6RIY
Crystal structure of MchDnaB-1 intein (N145AA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-09 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.310, 95.780, 35.860 |
| Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
| Resolution | 34.230 - 1.630 |
| R-factor | 0.1716 |
| Rwork | 0.169 |
| R-free | 0.21720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rix |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.802 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.230 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.081 | 0.501 |
| Number of reflections | 24075 | 1195 |
| <I/σ(I)> | 7.3 | 1.9 |
| Completeness [%] | 95.8 | 96.8 |
| Redundancy | 2.5 | 2.6 |
| CC(1/2) | 0.994 | 0.603 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris-HCl pH 7.5, magnesium chloride, polyethylene glycol 4000 |
