6RIA
Bactofilin from Thermus thermophilus, F105R mutant crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97623 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 191.866, 244.896, 505.917 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.887 - 3.500 |
| R-factor | 0.284 |
| Rwork | 0.283 |
| R-free | 0.30690 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.682 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.123 | 0.541 |
| Rpim | 0.070 | 0.309 |
| Number of reflections | 147502 | 84093 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.996 | 0.869 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.7 | 292 | 6.7-7.3 v/v % 2-propanol, 0.17-0.19 M lithium sulfate, 0.1 M phosphate citrate, pH 3.7 and 0.4 M ammonium acetate as an additive |
