6RHT
Structure of Pediococcus acidilactici putative lactate oxidase WT protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.980 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 135.240, 135.240, 124.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.900 - 1.900 |
| R-factor | 0.19145 |
| Rwork | 0.190 |
| R-free | 0.22093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.628 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.630 | 1.895 |
| High resolution limit [Å] | 1.900 | 1.928 |
| Number of reflections | 45969 | 2251 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.1 M Sodium Malonate, 0.1 M Hepes pH 7.0, 0.5% Jeffamine ED-2003 |
