6RHQ
Crystal Structure of Two-Domain Laccase mutant I170A from Streptomyces griseoflavus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00318 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.790, 94.690, 116.490 |
| Unit cell angles | 90.00, 90.87, 90.00 |
Refinement procedure
| Resolution | 49.607 - 1.980 |
| R-factor | 0.1761 |
| Rwork | 0.174 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o4q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.854 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmeas | 0.135 | 0.952 |
| Number of reflections | 112783 | 8272 |
| <I/σ(I)> | 10.13 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.72 | |
| CC(1/2) | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 296 | 25% Smear medium PEG, 0.1 M Bicine |
