6RH6

Solution structure and 1H, 13C and 15N chemical shift assignments for the complex of NECAP1 PHear domain with phosphorylated AP2 mu2 148-163

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE III		600
2	Bruker	AVANCE		600
3	Bruker	AVANCE III		800

Experiment

experiment id	conditions id	solution id	Experiment type
10	1	3	2D 1H-13C HSQC aliphatic (constant time)
11	1	3	2D 1H-13C HSQC aromatic (constant time)
12	1	3	2D 1H-1H NOESY (reject 15N,13C coupled 1H in F1, accept 13C coupled 1H in F2) tau(m) 150ms
13	1	2	3D HBHA(CO)NH
14	1	2	3D HNCACB
15	1	3	3D HCCH-COSY (1H,13C,1H)
16	1	3	3D HCCH-TOCSY (13C,13C,1H)
17	1	2	3D 1H-15N NOESY tau(m) 150ms
18	1	2	3D 1H-13C NOESY aliphatic tau(m) 150ms
19	1	3	3D 1H-13C NOESY aliphatic tau(m) 150ms
2	1	2	2D 1H-15N HSQC
20	1	2	3D 1H-13C NOESY aromatic tau(m) 150ms
21	1	3	3D 1H-13C NOESY aromatic tau(m) 150ms
22	1	2	3D 1H-13C NOESY aliphatic (reject 15N,13C coupled 1H in F1) tau(m) 150ms
23	1	3	3D 1H-13C NOESY aliphatic (reject 15N,13C coupled 1H in F1) tau(m) 150ms
24	1	2	3D 1H-13C NOESY aromatic (reject 15N,13C coupled 1H in F1) tau(m) 150ms
25	1	3	3D 1H-13C NOESY aromatic (reject 15N,13C coupled 1H in F1) tau(m) 150ms
3	1	2	2D 1H-13C HSQC aliphatic
4	1	2	2D 1H-13C HSQC aromatic
5	1	2	2D 1H-1H NOESY (reject 15N,13C coupled 1H in F1, accept 15N,13C coupled 1H in F2) tau(m) 150ms
6	1	2	2D 1H-1H NOESY (reject 15N,13C coupled 1H in F1 and F2) tau(m) 150ms
7	1	2	2D 1H-1H TOCSY (reject 15N,13C coupled 1H in F2)
8	1	3	2D 1H-13C HSQC aliphatic
9	1	3	2D 1H-13C HSQC aromatic

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	7	1	298

Conformers

Conformers Calculated Total Number	50
Conformers Submitted Total Number	30