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6RGO

Complex of KlAtg21 with coiled-coil of AgAtg16

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2016-08-08
DetectorDECTRIS PILATUS 6M
Wavelength(s)0.97918
Spacegroup nameP 32 2 1
Unit cell lengths123.447, 123.447, 185.168
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution46.290 - 3.701
R-factor0.2856
Rwork0.284
R-free0.30650
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5ltg
RMSD bond length0.010
RMSD bond angle1.502
Data reduction softwareMOSFLM
Data scaling softwareAimless (0.7.3)
Phasing softwarePHASER
Refinement softwarePHENIX ((1.14_3260: ???))
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]46.29046.2904.050
High resolution limit [Å]3.7009.0703.700
Rmerge0.2300.0451.697
Rmeas0.2390.0471.758
Rpim0.0620.0130.452
Total number of observations2544891761261136
Number of reflections1791713274231
<I/σ(I)>9.4432.1
Completeness [%]99.698.699.9
Redundancy14.213.314.4
CC(1/2)0.9990.9990.476
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5293.15Protein concetration 13 mg/mL, Clostripain for in situ proteolysis (1:2000 ratio). Reservoir: ProComplex (Molecular Dimension) B11: 15 % PEG4000, 0.1 M HEPES pH 7.5. Droplet: 2 microL Protein (mit 1:2000 Clostripain) + 3 microL Reservoir.

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