6RGO
Complex of KlAtg21 with coiled-coil of AgAtg16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 123.447, 123.447, 185.168 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.290 - 3.701 |
R-factor | 0.2856 |
Rwork | 0.284 |
R-free | 0.30650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ltg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.502 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.290 | 46.290 | 4.050 |
High resolution limit [Å] | 3.700 | 9.070 | 3.700 |
Rmerge | 0.230 | 0.045 | 1.697 |
Rmeas | 0.239 | 0.047 | 1.758 |
Rpim | 0.062 | 0.013 | 0.452 |
Total number of observations | 254489 | 17612 | 61136 |
Number of reflections | 17917 | 1327 | 4231 |
<I/σ(I)> | 9.4 | 43 | 2.1 |
Completeness [%] | 99.6 | 98.6 | 99.9 |
Redundancy | 14.2 | 13.3 | 14.4 |
CC(1/2) | 0.999 | 0.999 | 0.476 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | Protein concetration 13 mg/mL, Clostripain for in situ proteolysis (1:2000 ratio). Reservoir: ProComplex (Molecular Dimension) B11: 15 % PEG4000, 0.1 M HEPES pH 7.5. Droplet: 2 microL Protein (mit 1:2000 Clostripain) + 3 microL Reservoir. |