6RGM

urate oxidase under 130 bar of krypton

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.861
Spacegroup name	I 2 2 2
Unit cell lengths	78.250, 94.940, 104.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.140 - 1.500
R-factor	0.14177
Rwork	0.140
R-free	0.16949
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6ic1
RMSD bond length	0.013
RMSD bond angle	1.775
Data reduction software	cctbx.xfel
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.140	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.055	0.580
Rpim	0.017	0.210
Number of reflections	62225	8901
<I/σ(I)>	21.5	3.4
Completeness [%]	99.5	98.4
Redundancy	13.3	12.4
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	291	20 microliters of protein+azaxanthin, pH 8 TRIS 0.05M mixed with 20 microliters TRIS 0.05M + 10 % (v:v) PEG 8000.