6RFC
Crystal structure of the potassium-pumping G263F mutant of the light-driven sodium pump KR2 in the monomeric form, pH 4.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 40.769, 81.964, 232.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.1792 |
Rwork | 0.177 |
R-free | 0.22750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xtl |
RMSD bond length | 0.004 |
RMSD bond angle | 0.769 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.3) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.500 | 40.500 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.048 | 0.018 | 0.405 |
Rmeas | 0.055 | 0.022 | 0.466 |
Rpim | 0.027 | 0.012 | 0.226 |
Total number of observations | 106312 | 1238 | 8269 |
Number of reflections | 26858 | 349 | 1983 |
<I/σ(I)> | 14.8 | 36.6 | 3.2 |
Completeness [%] | 99.5 | 97.5 | 100 |
Redundancy | 4 | 3.5 | 4.2 |
CC(1/2) | 0.999 | 0.999 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 2.0 M Sodium Malonate, pH 4.3 |