6RCU
PfRH5 bound to monoclonal antibodies R5.004 and R5.016
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 235.150, 58.784, 116.794 |
Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
Resolution | 46.920 - 4.005 |
R-factor | 0.2874 |
Rwork | 0.285 |
R-free | 0.33900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.188 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.920 | 4.070 |
High resolution limit [Å] | 4.005 | 4.005 |
Number of reflections | 13131 | 522 |
<I/σ(I)> | 5.9 | |
Completeness [%] | 99.0 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.15 M DL malic acid, 20% PEG 3350 |