6RCS

PfRH5-binding monoclonal antibody R5.016

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92819
Spacegroup name	P 43 21 2
Unit cell lengths	80.080, 80.080, 162.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.240 - 2.100
R-factor	0.205
Rwork	0.204
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.070	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	31807	1564
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M lithium sulfate, 0.1 M NaAc pH 4.5, 50% PEG 400