6RA8

Horse spleen apoferritin under 2 kBar of argon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.771
Spacegroup name	F 4 3 2
Unit cell lengths	180.873, 180.873, 180.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.260 - 2.000
R-factor	0.1736
Rwork	0.171
R-free	0.22111
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2w0o.pdb
RMSD bond length	0.007
RMSD bond angle	1.332
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	FFT
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.260	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.102	0.679
Rpim	0.012	0.086
Number of reflections	17689	2516
<I/σ(I)>	40.1	8
Completeness [%]	100.0	99.7
Redundancy	67.6	61.9
CC(1/2)	1.000	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Ammonium sulfate 0.6 M + cadmium sulfate 0.1 M