6RA0

A ubiquitin-like dimerization domain controls protein kinase D activation by trans-autophosphorylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97626
Spacegroup name	P 21 21 2
Unit cell lengths	45.413, 83.018, 37.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.510 - 2.260
R-factor	0.2339
Rwork	0.232
R-free	0.27620
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.071
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.260	2.260
Number of reflections	6939	641
<I/σ(I)>	11.2
Completeness [%]	99.4
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	6.8% PEG8000 18% ethylene glycol 0.1 M MES, pH 6.5 0.03 M NaF 0.03 M NaBr 0.03 M NaI
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	6.8% PEG8000 18% ethylene glycol 0.1 M MES, pH 6.5 0.03 M NaF 0.03 M NaBr 0.03 M NaI