6R9R

Crystal structure of Csx1 in complex with cyclic oligoadenylate cOA4 conformation 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 21 21 21
Unit cell lengths	104.480, 119.520, 358.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.240 - 2.700
R-factor	0.193
Rwork	0.192
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qzt
RMSD bond length	0.009
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.620	2.880
High resolution limit [Å]	2.700	2.700
Number of reflections	44353	2218
<I/σ(I)>	17.7	1.04
Completeness [%]	93.5
Redundancy	8.8	8.7
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES PH=7.5, 20 MM LI2SO4, 30% PEG 600, 10% GLYCEROL