6R9P
Structure of Saccharomyces cerevisiae apo Pan2 pseudoubiquitin hydrolase-RNA exonuclease (UCH-Exo) module in complex with AAUUAA RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9159 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 91.885, 117.091, 255.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.478 - 2.980 |
R-factor | 0.2635 |
Rwork | 0.261 |
R-free | 0.30860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4czw |
Data reduction software | XDS |
Data scaling software | xia2 (1.12) |
Phasing software | PHASER (2.8) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.450 | 3.060 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.065 | 1.512 |
Number of reflections | 28662 | 4551 |
<I/σ(I)> | 13.5 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 | 6.21 |
CC(1/2) | 0.999 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.4 M ammonium phosphate |