6R9D
Crystal structure of an asymmetric dimer of the N-terminal domain of Euprosthenops australis Major Ampullate Spidroin 1 (dragline silk)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.93340 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 126.631, 65.114, 95.968 |
Unit cell angles | 90.00, 91.85, 90.00 |
Refinement procedure
Resolution | 57.900 - 2.100 |
R-factor | 0.2093 |
Rwork | 0.208 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lr2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.453 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.900 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.195 | 0.422 |
Rmeas | 0.213 | 0.559 |
Rpim | 0.085 | 0.364 |
Number of reflections | 45467 | 4464 |
<I/σ(I)> | 10.16 | 2.39 |
Completeness [%] | 99.3 | 98.13 |
Redundancy | 4.8 | 2.2 |
CC(1/2) | 0.993 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% PEG 3350 1 M Ammonium sulphate 0.1 M Tris-HCl buffer pH 8.5 15% PEG 400 |