6R99

Crystal Structure of Human CLN protein 5 (Ceroid Lipofuscinosis Neuronal Protein 5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	58.420, 58.420, 179.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.046 - 2.700
R-factor	0.222
Rwork	0.221
R-free	0.24710
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.727
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.046	44.046	2.720
High resolution limit [Å]	2.650	11.860	2.650
Rmerge	0.229	0.078	1.294
Rmeas	0.233	0.079	1.362
Total number of observations	550105
Number of reflections	19772	217	1412
<I/σ(I)>	13.53	46.16	1.55
Completeness [%]	100.0	99.1	100
Redundancy	27.822	33.401	10.176
CC(1/2)	0.999	1.000	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, magnesium nitrate