6R8N
STRUCTURE DETERMINATION OF THE TETRAHEDRAL AMINOPEPTIDASE TET2 FROM P. HORIKOSHII BY USE OF COMBINED SOLID-STATE NMR, SOLUTION-STATE NMR AND EM DATA 4.1 A, FOLLOWED BY REAL_SPACE_REFINEMENT AT 4.1 A
Spectrometer
Experimental method: ELECTRON MICROSCOPY, SOLUTION NMR
Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
2 | Bruker | AVANCE II | 600 | |
3 | Varian | INOVA | 800 | |
4 | Varian | INOVA | 600 |
Experiment
experiment id | conditions id | solution id | Experiment type |
1 | 1 | 1 | RFDR 3D |
10 | 1 | 5 | hCANH |
11 | 1 | 5 | hCONH |
12 | 1 | 5 | hcoCAcoNH |
2 | 3 | 3 | DARR |
3 | 2 | 2 | 3D 1H-13C NOESY aliphatic |
4 | 3 | 4 | CCdream (CC) |
5 | 3 | 4 | NCO |
6 | 3 | 4 | NCOCX |
7 | 3 | 4 | NCACX |
8 | 3 | 4 | CONCACB |
9 | 3 | 4 | CANCOCX |
NMR Sample
conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
1 | 7.5 | 1 | 300 |
2 | 8 | 1 | 328 |
3 | 7.5 | 1 | 300 |
Conformers
Conformers Calculated Total Number | 100 |
Conformers Submitted Total Number | 1 |
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.1 |
Number of Particle | 30407 |
Software | RELION,X-PLOR,Latitude,CTFFIND,iMODFIT |
Symmetry | T |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |