6R8I

PP4R3A EVH1 domain bound to FxxP motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	60.586, 60.586, 91.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.841 - 1.517
R-factor	0.1905
Rwork	0.189
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wsf
RMSD bond length	0.006
RMSD bond angle	0.908
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.840	1.590
High resolution limit [Å]	1.520	1.520
Number of reflections	50548	8189
<I/σ(I)>	22.8
Completeness [%]	99.7
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1 M tri-sodium citrate, 0.1 M sodium cacodylate pH 6.5